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Title   배양세포에서 Ga-67 섭취기전에 관한 연구 : Transferrin 의존성 및 비의존성 섭취기전 ( Uptake of Ga-67 by Cultured Cells : Transferrin - dependent and Transferrin - independent Mechanisms )
Publicationinfo   2000 Jan; 032(04): 742-750.
Key_word   Transferrin, Mechanism, Cultured cells, Transformation
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Abstract   Purpose: We determined whether the uptake of Ga-67 by cultured cells occur by both transferrin (Tf)-dependent and independent mechanisms and the mechanism and magnitude of its uptake may vary as the degree of expression of the transformed phenotype. Materials and Methods: Uptake of Ga-67 between the tansformed and untransformed cells was compared. Cells were incubated with Ga-67 in either the presence or absence of Tf and with complete medium containing Ga-67 after preincubating with anti-Tf receptor antibodies at 37"C in 8% CO2. Monolayers of cells were washed and trypsinized. Radioactivity and protein content of the samples were determined. Results: Uptake of Ga-67 by cultured cells occurred both in Tf-bound and ionic form and was increased with radioactivity and time. The magnitude for the uptake of Tf-bound form was approximately 3 and 6-fold greater than ionic form. In the presence of Tf, uptake of Ga-67 was 2-fold greater in the transformed cells. Conversely, In the absence of Tf, it was 1.5-fold greater in the untransformed cells. Regardless of blocking the Tf receptor by anti-Tf receptor antibodies, a significant amount of intracellular Ga-67 uptake was found. Conclusion: Dual mechanisms exist for the uptake of Ga-67 by cultured cells. The primary important one was the Tf-dependent system. Tf-dependent and independent mechanisms and the magnitude operated oppositely in the transformed cells when compared to their untransformed counterpart.
저 자   손명희(Myung Hee Sohn),임석태(Seok Tae Lem),곽재용(Jae Yong Kwak),임창열(Chang Yeol Yim)